Meta-Topolin mediated in vitro propagation in an ornamentally important crop Iris × hollandica Tub. cv. professor Blaauw and genetic fidelity studies using SCoT markers

Dutch iris is a commercially important bulbous ornamental crop. Its high demand in global floriculture market necessitates the production of its high-quality planting material. In present investigation, an efficient vitro propagation system has been developed for Iris × hollandica Tub. cv. Professor Blaauw (Dutch iris) using meta-Topolin (mT) first time. Effect various concentrations BAP, Kn, and mT (0, 0.5, 1.0, 1.5 2 mg L−1) along with varying photoperiods (16 h light dark incubation 1, 2, 3 4 weeks) on shoot induction from twin scale explants was studied. Of cytokinins tested, different doses resulted better response than BAP Kn. Photoperiod duration also affected significantly. Along 1 week, cytokinin at 1.0 L−1 MS medium maximum (91.63%) increased emergence micro shoots (4.83 shoots/explant average length 5.02 cm). Efficacy alone or combination auxins multiplication compared. The synergistic effect cytokinin-auxin comprising + 0.25 NAA considerably higher number (17.53) mean (7.06 cm) bulblets (2.74). Positive sucrose concentration (90 g paclobutrazol (5 bulblet formation size observed respectively. superiority over found during rhizogenesis. Shoots raised were healthy long enough, thus showed rooting (63.83%) ½ 0.5 IAA after weeks incubation. About 89.16% survival rate recorded plantlets under ex conditions. Analysis clonal fidelity thirteen regenerated plants done SCoT markers. Out 36 primers, 13 primers clearly scorable monomorphic bands, displaying genetic uniformity among plantlets. This mediated protocol can be routinely used rapid large this valuable Reliable consistent regeneration established, which applied crop will open up way engineering/gene editing other crops.